Uonita Khachoomian, Stephanie C. Heard, Jeannette Dimple, and Jaclyn M. Winter Department of Medicinal Chemistry, College of Pharmacy, University of Utah
Infectious diseases cause 15 million annual deaths worldwide, and over the past few decades there has been a tremendous increase in infections that are caused by antibiotic-resistant Gram-positive and Gram-negative bacteria.1Natural products, also known as secondary metabolites, are small molecules produced in nature that have important pharmacological applications due to their broad biological activity. In their natural environment, microorganisms exist in complex communities, and specific intra- and interspecies communication can trigger activation of silent biosynthetic gene clusters leading to production of secondary metabolites. Pestalone is a fungal natural product produced byPestalotiasp. CNL-365 when it is co-cultured with the specific bacterial challenger CNJ-328, and it has potent antimicrobial activity against resistant bacterial strains. Pestalone harbors multiple regulatory genes in its biosynthetic cluster that play a significant role in its production. In addition to investigating the expression of several pestalone biosynthetic genes by using transcriptomics, this project also aimed to investigate the presence of these specific regulatory genes in other fungal strains in public databases.
1)Nii-Trebi N. I. (2017). Emerging and Neglected Infectious Diseases: Insights, Advances, and Challenges. BioMed research international, 2017, 5245021. doi:10.1155/2017/5245021.
Recommend0 recommendationsPublished in